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Journal: ACS Applied Bio Materials
Article Title: Differential Effects of Confinement on the Dynamics of Normal and Tumor-Derived Pancreatic Ductal Organoids
doi: 10.1021/acsabm.4c01301
Figure Lengend Snippet: Particle tracking quantifies microgel displacement due to organoid movement. (A) Schematic of single particle tracking with travel distance and linearity index defined. (B) Normal organoid in a confined microenvironment generates (B′) rotational trajectories. (C) Tumor organoid in a confined microenvironment generates (C′) linear trajectories. Particle trajectory (D) travel distances and (E) linearity indices (normal: n = 2306 trajectories, N = 13 organoids; tumor: n = 3090 trajectories, N = 17 organoids). Statistical significance was determined using an unpaired t test. **** p ≤ 0.0001. Error bars: 95% confidence intervals. Scale bar = 100 μm for composites and 10 μm for inset.
Article Snippet: Rheological properties of pure Matrigel (less confined) and microgel–Matrigel composites (more confined) were measured by using a
Techniques: Single-particle Tracking